sa β gal activity assay kit Search Results


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Beijing Solarbio Science sa-β-gal staining kit b8050
Sa β Gal Staining Kit B8050, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science sa-β-gal staining kit
Sa β Gal Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc sa-β-gal staining
Sa β Gal Staining, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc sa-β-gal staining cellular senescence assay kit
Sa β Gal Staining Cellular Senescence Assay Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science β-gal staining kit g1580

β Gal Staining Kit G1580, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime senescence β-galactosidase staining kit
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Senescence β Galactosidase Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science sa-b-gal staining kit solarbio, beijing, china
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Sa B Gal Staining Kit Solarbio, Beijing, China, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc fluorometric assay kit sa-β-gal
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Fluorometric Assay Kit Sa β Gal, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genmed Inc sa-β-gal staining kit
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Sa β Gal Staining Kit, supplied by Genmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EuroClone sa-β-gal staining kit bk9860s
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Sa β Gal Staining Kit Bk9860s, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime cytochemical staining kit of sa-β-gal
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Cytochemical Staining Kit Of Sa β Gal, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science histochemical staining kit of cellular sa-β-gal
miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation <t>of</t> <t>SA-β-gal</t> staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.
Histochemical Staining Kit Of Cellular Sa β Gal, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: iScience

Article Title: The role of mitofusin 2 in regulating endothelial cell senescence: Implications for vascular aging

doi: 10.1016/j.isci.2024.110809

Figure Lengend Snippet:

Article Snippet: β-Gal staining kit , Solarbio , G1580.

Techniques: Virus, Recombinant, Bicinchoninic Acid Protein Assay, Staining, Gene Expression, Western Blot, Plasmid Preparation, Software

miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation of SA-β-gal staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.

Journal: Cell Cycle

Article Title: miR-300 regulates cellular radiosensitivity through targeting p53 and apaf1 in human lung cancer cells

doi: 10.1080/15384101.2017.1367070

Figure Lengend Snippet: miR-300 reduces p53-dependent senescence induced by IR. (A) High dose irradiation actives cell cycle suspension in A549 cells. A549 cells were exposed to 10 Gy of X-rays, the cell cycle phase was analyzed at 24, 48 and 72 h post-irradiation. (B) miR-300 abolishes cell cycle suspension. The cell cycle phase of A549 cells transfected with miRNA mimics was analyzed at 48 h after irradiation. (C) Representative cell cycle distribution of cells in (B), the data were analyzed with IDEAS Application v6.0. (D) Growth curves of A549 cells treated with 10 Gy of X-rays. Cells were transfected with P300 or NC before irradiation. Graphs represent mean of a triplicate experiment, error bars represent SD. (E) Colonies were formed 8 days after irradiation in miR-300 overexpression (P300) or p53 inhibition (si-p53) group and photographed using a phase contrast microscope. The arrows indicate colonies. Scale bar, 100 μm. (F) Quantitation of SA-β-gal staining A549 cells with miR-300 overexpression (P300) or p53 inhibition (si-p53) 8 days after treated with 10 Gy of X-rays. Ctrl, control, cells exposed to IR but no transfection; NC, pre-miRNA negative control; P300, pre-miR-300; si-p53, p53 siRNA. *P < 0.05, ** P < 0.01, compared to Ctrl.

Article Snippet: Cells were stained by Senescence Associated (SA) β-Galactosidase Staining Kit (Beyotime, C0602) following the protocol 8 d after irradiation.

Techniques: Irradiation, Transfection, Over Expression, Inhibition, Microscopy, Quantitation Assay, Staining, Negative Control